supplemental Fig. S2.
Effects of S1P turnover on the colocalization of Plin5 with mitochondria in INS1E beta-cells. INS1E cells were incubated in the absence or presence of PA or OA (500 μM of each) for 24 h. Thereafter, mitochondrial network was analyzed after incubation with Mitotracker Deep RedTM (red) and cellular distribution Plin5 (green) was analyzed. Shown are representative pictures of n = 3 independent experiments. Asterixes: colocalization of Plin5 with mitochondria, arrows: Plin5-coated spherical structures not colocalized with mitochondria. Bars: 10 μm.