Fig. 6.

Effects of enhanced S1P turnover and FFA on peroxisomal and mitochondrial hydrogen peroxide production in insulin-secreting INS1E cells. INS1E cells were incubated in the absence or presence of PA or OA (500 μM of each) for 24 h. Peroxisomal hydrogen peroxide generation was estimated by expressing the fluorescence sensor HyPer-Peroxi (A) and mitochondrial by expressing HyPer-Mito (B) sensor proteins and evaluation of fluorescence shift by the CellSens software at the Olympus fluorescence microscope (representative pictures from n = 3 individual experiments). Arrows depict representative cells with higher red HyPer protein fluorescence. Bars: 10 μm.