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. 2024 Jun 29;65(8):100587. doi: 10.1016/j.jlr.2024.100587

Fig. 9.

Fig. 9

Effects of S1P turnover and free fatty acids on caspase-3/7 activation and lipid droplet formation in human EndoC-βH1 beta-cells. EndoC-βH1 beta-cells were incubated in the absence or presence of PA or OA (500 μM of each) for 24 h. Shown are (A) caspase-3/7 activation measured by Caspase-3/7-Glo assay (MEANS ± SEM from n = 6–10), (B) lipid droplet formation visualized by OilRed staining (representative pictures from n = 3), (C) representative Western blots for the detection of SPL, SGPP1, SGPP2, and SK2 from 3–4 independent experiments. ANOVA followed by Bonferroni, ∗P < 0.05, ∗∗P < 0.01, versus untreated, #P < 0.05, versus INS1E-ctr cells treated in the same way. Bars: 20 μm, inset bars: 2 μm.