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. 2024 Aug 17;43(4):910–922. doi: 10.1007/s10930-024-10221-0

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© His Majesty the King in Right of Canada as represented by the Minister of Agriculture and Agri-Food, and Liam Worrel 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Phylogenetic analysis of rumen carbohydrate esterase family 15 esterases. Glucuronoyl esterases in the carbohydrate esterase family 15 esterase family from the CAZy database (accessed August 10, 2023) and the rumen collection were clustered by their sequence identity, used as query sequence inputs for SACCHARIS [27], and embedded into a mid-point rooted phylogenetic tree. Coloured rings surrounding the tree highlight sequence characteristics by domain (inner) and EC number and CAZy database annotated function (outer). Carbohydrate esterase family 15 esterases from anaerobic gut fungi and rumen bacteria are highlighted in purple (inner ring) and red, respectively. Proteins that have been biochemically characterized are shown in orange (outer ring). Proteins characterized in this study are highlighted with a triangle on the outside of the circle. Sequences used to generate the tree are numbered and accession codes provided in Supplementary Data 1