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. 2024 Aug 17;43(4):910–922. doi: 10.1007/s10930-024-10221-0

Table 2.

Catalytic activity of FsCE15, PrCE15 and RfCE15 against Benzyl D-glucuronate

Enzyme pH optimum Specific activity (µmol min-1mg-1) Apparent Km (mM) Apparent kcat (s-1) Apparent kcat/ Km (s-1M-1)
PrCE15 7.5 12.5 ± 0.8 5.0 ± 0.4 22.3 ± 0.8 4485 ± 376
RfCE15 6.5 14.9 ± 0.3 6.0 ± 0.4 29.7 ± 1.0 4950 ± 392
FsCE15 8.0 2.66 ± 0.08 ND*, Reaction could not be saturated

*Not determined