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. 2024 Jul 11;300(8):107558. doi: 10.1016/j.jbc.2024.107558

Figure 5.

Figure 5

Effect of exogenous L-fucose on the expression levels of specific anti-OVA antibodies.A, mice were treated with the immunization schedule with OVA and administrated with L-fucose at 0.4 mg/g/day. Blood was collected on the 0, second, third, fourth, fifth, sixth, seventh week post immunization. B, the OVA-specific IgGs were extracted as described in “Experimental procedures”. The levels of OVA-specific IgGs in an equal amount of serum (2 μl) were detected by anti-mouse IgG antibody, and the levels of core fucosylated IgG were detected using LCA lectin blot analysis. CBB was used as a loading control. C, comparison of expression levels of the OVA-specific IgGs among Fut8+/+, Fut8+/−, and Fut8+/− mice treated with L-fucose. The data were obtained from at least three mice and analyzed by Image J using one-way ANOVA with Tukey’s post hoc analysis as the mean ± SEM. The relative levels of OVA-specific IgGs indicate the OVA-specific IgG levels versus the OVA-specific IgG levels at the second immunization point, which were set as 1.0. ∗∗p < 0.01; ∗∗∗p < 0.001. CBB, Coomassie brilliant blue; Fut8+/−, Fut8 heterozygous knockout; IgG, immunoglobulin G; LCA, Lens culinaris agglutinin; OVA, ovalbumin.