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. 2024 Jun 28;300(8):107514. doi: 10.1016/j.jbc.2024.107514

Table 1.

Expression of RNA polymerases

Sense Family Virus Expression system Construct Composition M.W. (kDa)a PDB
Positive ssRNA Coronaviridae SARS-CoV-2 BEVSb nsp5-↓c nsp7-↓ 8xHis-nsp8-↓nsp12d↓ Tetramer 165 7UO4e
MERS-CoV nsp5-↓ nsp7-↓ 8xHis-nsp8-↓nsp12-Strep Tetramer 165 n/a
Picornaviridae HRV-16 E. coli 8xHis-3Dpol Monomer 50 4K50
EV-71 50 3N6L
Nonsegmented negative
ssRNA		 Pneumoviridae RSV BEVS TEV-↓ 8xHis-P-↓L-Strep Dimer 282 6PZK
HMPV TEV-↓ 8xHis-P-↓ Strep-L 267 6U5O
Paramyxoviridae HPIV-3f BEVS p10P-pPHL-8xHis Dimer 323 8KDB
PIV-5f 298 6V85
Segmented negative ssRNA Orthomyxoviridae FluB BEVS TEV-↓ 8xHis-↓ PA-↓PB1-↓ PB2-Strep Trimer 263 6QCV
Arenaviridae LASV BEVS Strep-8xHis-L Monomer 257 7OJN
Human DNA-dependent RNA polymerase h-mtRNAP BEVS Strep-8xHis-RNAP Monomer 138 4BOC

TEV, tobacco etch virus protease.

a

Molecular weight is an approximation and calculated according to the subunit composition of the replication complex (e.g. coronavirus replication complex accounts for the ratio 1:2:1 of Nsp7:Nsp8:Nsp12 as observed structurally). The protease is not included in the calculation.

b

Baculovirus expression vector system.

c

Down arrow represents the cleavage site acted on by that constructs respective protease (underlined).

d

Bolded domains indicate where the RdRp motifs responsible for catalyzing RNA synthesis are contained.

e

Ternary structure of the SARS-CoV-2 replication complex with GS-443902 in the active site as reported by Malone et al. (25).

f

Cloned into pFastBac-DUAL vector employing the p10 and polyhedrin (pPH) promoter as reported by Abdella at el (40).