Table 3.

The SHERLOCK system.

Component	Volume
RPA3-F(100μM)	0.48 μL
RPA3-R(100μM)	0.48 μL
Rehydration buffer	59 μL
Lw Cas13a protein	5.91 μL
crRNA	4 μL
RNA reporter (5′-FAM-UUUUU-BHQ-3′)	6 μL
RNase inhibitor	2.73 μL
NTP buffer mix	8 μL
T7 RNA polymerase mix	2.5 μL
1 M MgCl2	0.9 μL
MgAc	5 μL
DNA	5 μL
Total	100 μL