HiS-PGCs maintain a higher level of expression of cell migration-related genes. (A) Comparative Analysis of normalized FPKM of cell migration genes between HiS-PGCs and FAcs-PGCs. (B) Heatmap of cell migration genes between HiS-PGCs and FAcs-PGCs. (C) GSEA of cell migration genes at HiS-PGCs versus FAcs-PGCs. (D) Representative images showing the migration of HiS-PGCs and FAcs-PGCs to recipient chicken embryonic gonads. Scale bar: 250μm. qRT-PCR analysis of OVOL3 (E), SEMA4G (F), RET (G), and LAMA5 (H) gene between HiS-PGCs and FAcs-PGCs. FPKM comparison analysis of OVOL3 (I), SEMA4G (J), RET (K), and LAMA5 (L) gene between HiS-PGCs and FAcs-PGCs. ** indicates p < 0.01. *** indicates p < 0.001.