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. Author manuscript; available in PMC: 2024 Aug 26.
Published in final edited form as: Dev Cell. 2023 Jan 27;58(3):211–223.e5. doi: 10.1016/j.devcel.2023.01.004

Figure 6. PCs sense and react to non-local BM changes through focal adhesion signaling.

Figure 6.

(A) Bursting assay of BM fibril increase in the central follicle epithelium (mirr>EHBP1OE, n=34). Red arrow indicates burst position.

(B-C) Overexpressing ColIV (B, green, n=20) or EHBP1 (C, n=25) in the central follicle causes anterior PCs to upregulate MMP1 (magnified in insets). Non-ice preps.

(D) Depleting ColIV in the central follicle causes anterior PCs to downregulate MMP1 (n=6). Ice prep.

(E-F) MMP1 expression when central follicle ColIV depletion is combined with blocking focal adhesion signaling (vinc mutant clones, not expressing RFP) in either PCs and epithelium (E, n=11), or in epithelium alone (F, n= 15). Ice preps.