Fig. 1.

CREG1 deficiency aggravated DOX-induced cardiotoxicity by increasing myocardial ferroptosis

A-B. EF% and FS% in the Creg1-CKO mice and Creg1^fl/fl mice after DOX treatment (n = 6 for the control group, n = 8 for the DOX group). C-D. HE staining, Masson’s trichrome staining, and WGA staining in the Creg1-CKO mice and Creg1^fl/fl mice after DOX treatment (n = 3). E-F. Transmission electron microscope for mitochondria in the myocardium of Creg1-CKO mice and Creg1^fl/fl mice after DOX treatment (n = 3). G-H. Western blotting of CREG1 and ferroptosis-related proteins in the Creg1-CKO mice and Creg1^fl/fl mice after DOX treatment (n = 4). DOX: doxorubicin, Creg1-CKO: Creg1 cardiac-specific knockout mice; Creg1^fl/fl mice: littermate control mice. *p < 0.05, **p < 0.01 vs. Creg1^fl/fl mice. ^#p < 0.05, ^##p < 0.01 vs. Creg1-CKO mice; ^&p < 0.05, ^&&p < 0.01 vs. Creg1^fl/fl-DOX mice.