Fig. 5.

CREG1 overexpression attenuated cardiomyocytes ferroptosis by inhibiting PDK4 expression

A. Heatmap of differential genes in the myocardium of Creg1-TG-DOX and WT-DOX mice. B-D. Real-time PCR and western blotting of PDK4 in the myocardium of Creg1-TG-DOX and WT-DOX mice (n = 3). E-G. Real-time PCR and western blotting of PDK4 expression in CREG1-overexpressed NMCMs (n = 3). H–I. Effects of CREG1 overexpression and PDK4 overexpression on the expression of ferroptosis-related proteins in NMCMs, as determined by western blotting (n = 3). J-K. Effects of PDK4 overexpression on mitochondrial oxidation in the CREG1-overexpressed NMCMs using by MitoSOX staining (n = 5). L-M. Effects of CREG1 overexpression and PDK4 overexpression on mitochondrial membrane potential in NMCMs using JC-1 staining (n = 3). DOX: doxorubicin, NMCMs: neonatal mouse cardiomyocytes. **p < 0.01 vs. adcon group or WT-DOX group or adcon+DOX group; ^##p < 0.01 vs. adCREG1+DOX group; ^&p < 0.05, ^&&p < 0.01 vs. adPDK4+DOX group.