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. 2024 Jul 29;75:103293. doi: 10.1016/j.redox.2024.103293

Fig. 6.

Fig. 6

CREG1 inhibited the mRNA and protein expression of PDK4 in cardiomyocytes by regulating FOXO1

A. Transcription factor prediction of Pdk4 gene by JASPAR website.B–C. Western blotting of FOXO1, HOXA5 and STAT3 protein expression in CREG1-overexpressed NMCMs (n = 3). D-F. Effects of FOXO1 overexpression on the mRNA and protein of CREG1 and PDK4 in NMCMs (n = 3). G-I. Effects of FOXO1 knockdown on the mRNA and protein of CREG1 and PDK4 in NMCMs (n = 3). J. Double luciferase reporter gene array in HEK293T cells (n = 6). K. Effects of CREG1 overexpression on the Foxo1 mRNA level in NMCMs (n = 3). L-O. Effects of CREG1 overexpression on FOXO1 protein level under CQ or MG132 treatment. NMCMs: neonatal mouse cardiomyocytes. CQ: chloroquine (10 μM). **p < 0.01 vs. adcon or si-control or Pdk4 pro (WT) or pc-control group; ##p < 0.01 vs. adCREG1 group.