Table 7.
Non-alcoholic fatty liver disease activity score criteria percentage frequency of high-fructose diet-fed female rats
| Criteria | Steatosis | Hypertrophy | Inflammation | Total NAS | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Microvesicular | Macrovesicular | ||||||||||||||||||
| Score | 0 | 1 | 2 | 3 | 0 | 1 | 2 | 3 | 0 | 1 | 2 | 3 | 0 | 1 | 2 | 3 | <2 | 3-4 | ≥ 5 |
| PC + PW | 100 | 0 | 0 | 0 | 100 | 0 | 0 | 0 | 75 | 25 | 0 | 0 | 50 | 50 | 0 | 0 | 100 | 0 | 0 |
| PC + FS | 0 | 25 | 75 | 0 | 0 | 25 | 50 | 25 | 50 | 25 | 25 | 0 | 0 | 25 | 75 | 0 | 50 | 0 | 50 |
| FF + FS | 25 | 75 | 0 | 0 | 100 | 0 | 0 | 0 | 75 | 25 | 0 | 0 | 25 | 75 | 0 | 0 | 75 | 25 | 0 |
| Bst + PW | 100 | 0 | 0 | 0 | 100 | 0 | 0 | 0 | 100 | 0 | 0 | 0 | 50 | 50 | 0 | 0 | 100 | 0 | 0 |
| Bst + FS | 0 | 100 | 0 | 0 | 100 | 0 | 0 | 0 | 75 | 25 | 0 | 0 | 25 | 75 | 0 | 0 | 100 | 0 | 0 |
PC + PW = plain gelatine cubes + plain tap water to drink; PC + FS = plain gelatine cubes + 20% (w/v) fructose solution to drink; FF + FS = gelatine cube containing fenofibrate at a dose of 100 mg/kg body mass per day + fructose solution to drink. Bst + PW = gelatine cube containing β-sitosterol at a dose of 20 mg/kg body mass per day + plain tap water to drink; Bst + FS = gelatine cube containing β-sitosterol at a dose of 20 mg/kg body mass per day + fructose solution to drink (w/v);. n = 4 per treatment. NAS criteria: steatosis grade scoring 0: 0–5%; 1: 5–33%; 2: 33–66%; 3; > 66%; foci of lobular inflammation scoring 0: none; 1: 1–2; 2: >2. Hypertrophy/ballooning scoring 0: none; 1:1–2; 2: >2. Total NAS score interpretation: <2 = not steatohepatitis; 3–4 = uncertain; ≥ 5 = steatohepatitis