KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| c13G8 | Mishra et al.28 | N/A |
| Monoclonal anti-GP38 patient derived antibodies | This paper | N/A |
| Anti-CCHFV nucleocapsid mAb clone 9D5 | BEI Resources | Cat. #NR-40270 |
| Goat anti-human Fc HRP | Invitrogen | Cat. #A18817 |
| Anti-Human CD3 PerCp-Cy5.5 (clone UCHT1) | BioLegend | Cat. #300430; RRID: AB_893299 |
| Anti-Human CD8 PerCp-Cy5.5 (clone SK1) | BioLegend | Cat. #344710; RRID: AB_2044010 |
| Anti-Human CD14 PerCp-Cy5.5 (clone 61D3) | Invitrogen | Cat. #45-0149-42; RRID: AB_1518736 |
| Anti-Human CD16 PerCp-Cy5.5 (clone B73.1) | BioLegend | Cat. #360712; RRID: AB_2562955 |
| Anti-Human CD19 PE-Cy7 (clone HIB19) | BioLegend | Cat. #302216; RRID: AB_314246 |
| Anti-Human CD27 BV510 (clone M-T271) | BD Biosciences | Cat. #740167; RRID: AB_2739920 |
| Anti-Human IgM BV711 (clone UCH-B1) | BD Biosciences | Cat. #747877; RRID: AB_2872339 |
| Anti-Human IgD BV421 (clone IA6-2) | BioLegend | Cat. #348226; RRID: AB_2561619 |
| Anti-Human IgG BV605 (clone G18-145) | BD Biosciences | Cat. #563246; RRID: AB_2738092 |
| Anti-human IgA DyLight® 488 (clone EPR5367-76) | Abcam | Cat. #ab98553; RRID: AB_10672542 |
| Anti-human CD20 PE-Cy7 (clone 2H7) | BioLegend | Cat. #302311; RRID: AB_314259 |
| Anti-human IgM AF488 (MHM-88) | BioLegend | Cat. # 314533; RRID: AB_2566486 |
| Goat Anti-human Fc Antibody | Jackson ImmunoResearch | Cat. #109-001-008; RRID: AB_2337530 |
| Anti-CCHFV pre-Gn mAb clone 13G8 | BEI Resources | Cat. #NR-40294 |
| Anti-CCHFV pre-Gn mAb clone 7F5 | BEI Resources | Cat. #NR-40281 |
| Anti-CCHFV pre-Gn mAb clone 8F10 | BEI Resources | Cat. #NR-40282 |
| Goat F(ab')2 Anti-Human Kappa FITC | SouthernBiotech | Cat. #2062-02; RRID: AB_2795737 |
| Goat F(ab')2 Anti-Human Lambda FITC | SouthernBiotech | Cat. #2072-02; RRID: AB_2795767 |
| Anti-HA.11 Epitope Tag Antiobdy APC (clone 16B12) | BioLegend | Cat. #901523; RRID: AB_2734657 |
| Goat F(ab')2 anti-human IgG PE | SouthernBiotech | Cat. #2042-09; RRID: AB_2795662 |
| Bacterial and virus strains | ||
| CCHFV-IbAr10200 | USAMRIID | Institute stock |
| CCHFV-Afg09-2990 | USAMRIID | Institute stock |
| CCHFV-Turkey2004 | USAMRIID | Institute stock |
| CCHFV-Oman-199809166 | USAMRIID | Institute stock |
| Biological samples | ||
| PBMCs from CCHFV convalescent donors | This paper | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| In-Fusion enzyme | Takara Bio | Cat. #638949 |
| Pierce IgG elution buffer | ThermoFisher Scientific | Cat. #21009 |
| Lys-C Endoproteinase | ThermoFisher Scientific | Cat. #90051 |
| cOmplete™ Protease Inhibitor Cocktail tablet | Roche | Cat. #4693132001 |
| Polyethylenimine (PEI 25K™) | Polysciences | Cat. #23966-1 |
| Kifunensine | GlycoSyn | Cat. #FC-034 |
| rGP38 (CCHFV IbAr10200) | Mishra et al.28 | N/A |
| rGP38 (CCHFV Oman) | This Paper | N/A |
| rGP38 (CCHFV Kosova-Hoti) | This Paper | N/A |
| rGP38 (CCHFV Turkey2004) | This Paper | N/A |
| rGP38 (CCHFV Afg09) | This Paper | N/A |
| rGP38 (CCHFV M18-China) | This Paper | N/A |
| KPL Blue Sure Substrate | Seracare | Cat. #52-00-01 |
| Streptactin-PE | IBA Lifesciences | Cat. #6-5000-001 |
| Streptactin-APC | IBA Lifesciences | Cat. #6-5010-001 |
| Propidium Iodide | Invitrogen | Cat. #P3566 |
| 10% NP-40 | Thermo Scientific | Cat. #85124 |
| RNaseOUT | Invitrogen | Cat. #10777019 |
| SuperScript III 5X First Strand Reaction Buffer | ThermoFisher Scientific | Cat. #18057018 |
| SuperScript III Reverse Transcriptase | ThermoFisher Scientific | Cat. #18080044 |
| HotStartTaq Plus Polymerase | QIAGEN | Cat. #203646 |
| Yeast Extract-Peptone-Dextrose Medium | Gietz and Woods75 | N/A |
| Salmon Sperm DNA | Invitrogen | Cat. #15632011 |
| Polyethylene Glycol 3350 | Sigma Aldrich | Cat. #202444 |
| Lithium Acetate | Sigma Aldrich | Cat. #517992 |
| Streptavidin Alexa Fluor 633 | Invitrogen | Cat. #S11223 |
| Soluble Cytosolic Protein | Xu et al.76 | N/A |
| Soluble Membrane Protein | Xu et al.76 | N/A |
| ExtraAvidin-R-PE | Sigma | Cat. #E4011 |
| Critical commercial assays | ||
| MACS B Cell Isolation Kit II, Human | Miltenyi Biotec | Cat. #130-091-151 |
| Streptavidin Biosensors | Sartorius | Cat. #18-5021 |
| Anti-Human IgG Fc Capture Biosensors | Sartorius | Cat. #18-5064 |
| Anti-Mouse IgG Fc Capture Biosensors | Sartorius | Cat. #18-5090 |
| Octet Kinetics Buffer 10x | Sartorius | Cat. #18-1105 |
| GeneMorph II Random Mutagenesis Kit | Agilent | Cat. #200550 |
| EZ-Link Sulfo-NHS-LC Biotin | ThermoFisher Scientific | Cat. #A39257 |
| Deposited data | ||
| CCHFV IbAr10200 GP38 | Mishra et al.28 | PDB: 6VKF |
| Model of CCHFV GP38 bound to ADI-46143 Fab | This Paper | PDB: 8VVK |
| Model of CCHFV GP38 bound to c13G8 Fab | This Paper | PDB: 8VVL |
| Model of CCHFV GP38 bound to ADI-46152 and ADI-58048 Fabs | This Paper | PDB: 8VWW |
| CryoEM map of CCHFV GP38 bound to ADI-46152 and ADI-58048 Fabs | This Paper | EMDB: EMD-43604 |
| CryoEM map of CCHFV GP38 bound with ADI-46143 and ADI-46158 Fabs | This Paper | EMDB: EMD-43551 |
| CryoEM map of CCHFV GP38 bound with ADI-58062 and ADI-63530 Fabs | This Paper | EMDB: EMD-43552 |
| CryoEM map of CCHFV GP38 bound with ADI-58026 and ADI-63547 Fabs | This Paper | EMDB: EMD-43553 |
| Experimental models: Cell lines | ||
| ExpiCHO Expression System Kit | Gibco | Cat. #A29133 |
| FreeStyle 293-F cells | Gibco | Cat. #R79007 |
| Vero E6 | ATCC | Cat. #CRL-1586; RRID:CVCL_XD71 |
| Vero | ATCC | Cat. #CCL-81; RRID: CVCL_0059 |
| S. cerevisiae: Strain background: EBY100 | ATCC | Cat. #MYA-4941 |
| Experimental models: Organisms/strains | ||
| Mouse: B6.129S(Cg)-Stat1tm1Dlv/J | Jackson Labs | Cat. #012606 |
| Mouse: Type 1 interferon a/β receptor KO | Jackson Labs | RRID:MMRRC Strain 032405-JAX |
| Oligonucleotides | ||
| Single B Cell Primers for PCR | Tiller et al.77 | N/A |
| Recombinant DNA | ||
| Plasmid: pCAGGS-NP | Zivcec et al.45 | N/A |
| Plasmid: pCAGGS-L | Zivcec et al.45 | N/A |
| Plasmid: pCAGGS-GPC | Zivcec et al.45 | N/A |
| Plasmid: pSMART-LCK-Luc (minigenome) | Zivcec et al.45 | N/A |
| Plasmid: pCAGGS-T7 | Zivcec et al.45 | N/A |
| Software and algorithms | ||
| CCP4 | Winn et al.78 | RRID:SCR_007255 |
| GraphPad Prism Software V9.5.1 | GraphPad Software | https://graphpad.com/ |
| iMosflm | Battye et al.79 | RRID:SCR_014217 |
| AIMLESS | Evans and Murshudov80 | RRID:SCR_015747 |
| Phaser | McCoy et al.81 | RRID:SCR_014219 |
| Coot | Emsley and Cowtan82 | RRID:SCR_01422 |
| Phenix | Adams et al.83 | RRID:SCR_014224 |
| PyMOL | DeLano et al.84 | RRID: SCR_000305 |
| cryoSPARC | Punjani et al.85 | RRID:SCR_016501 |
| ISOLDE | Tristan Croll86 | https://tristanic.github.io/isolde/ |
| AlphaFold Protein Structure Database | Varadi et al.87 | RRID:SCR_023662 |
| DeepEMhancer | Sanchez-Garcia et al.88 | https://github.com/rsanchezgarc/deepEMhancer |
| PDBePISA | Krissinel and Henrick89 | RRID:SCR_015749 |
| UCSF ChimeraX | Goddard et al.90 | RRID:SCR_015872 |
| FlowJo | FlowJo/BD Biosciences | RRID:SCR_008520 |
| FCS Express | De Novo Software | RRID:SCR_016431 |
| Fortébio Octet Data Analysis Software V11.1 | Sartorius | RRID:SCR_023267 |
| Carterra LSA Kinetics Software V1.7.3055 | Carterra | https://carterra-bio.com/applications/kinetics-software/ |
| Bio-Rad CFX Manager 3.1 | Bio-Rad | Cat. # 1845000; RRID:SCR_017251 |
| Other | ||
| HiTrap MabSelect SuRe affinity column | Cytiva | Cat. #11-0034-94 |
| CaptureSelect™ IgG-CH1 affinity resin | ThermoFisher Scientific | Cat. #194320010 |
| Pierce™ Protein A Plus Agarose resin | ThermoFisher Scientific | Cat. #22812 |
| HisPur™ Ni-NTA Resin | Thermo Scientific | Cat. # 88223 |
| HiLoad 16/600 Superdex 200 column | Cytivia | Cat. #28-9893-35 |
| Superdex 200 Increase 10/300 GL | Cytiva | Cat. #28-9909-44 |
| CCHFV Oman-199809166 sequence | UniProt | Accession # A0A0U3C6Q7 |
| CCHFV Kosova-Hoti sequence | UniProt | Accession #B2BSL7 |
| CCHFV 200406546-Turkey sequence | UniProt | Accession # A0A0U2SQZ0 |
| CCHFV Afg09-2990 sequence | UniProt | Accession #E5FEZ4 |
| CCHFV 79121M18 sequence | UniProt | Accession #D4NYK3 |
| PELCO easiGlow™ Glow Discharge Cleaning System | Ted Pella | Cat. #91000 |
| Vitrobot Mark IV | ThermoFisher Scientific | N/A |
| Expifectamine CHO Transfection Kit | ThermoFisher Scientific | Cat. #A29129 |
| Quantifoil R 1.2/1.3 300 Mesh, Cu | Electron Microscopy Sciences | Cat. #Q350CR1.3 |