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. 2024 May 27;20(9):2076–2091. doi: 10.1080/15548627.2024.2356490

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 3. — Assessing the top p-S65-Ub antibodies in ELISA using recombinant protein. (A) Sandwich ELISA results for the detection of recombinant K48 (solid line) and K63 (dash line) p-S65-Ub tetramers (Ub4) that were serially diluted to the range of 1–1,000,000 pg/ml. (B) Direct ELISA results for the detection of p-S65-PRKN and nonphosphorylated PRKN recombinant proteins that were serially diluted to the range of 1–1,000,000 pg/ml. N = 2. Data are shown as mean with SD. ref. Ab: reference antibody.