Table 2.
Comparison of MSD ELISA detection of p-S65-Ub with different testing materials.
| Fold change in MSD ELISA detection ± range | ref. Ab | 11H2K3 | 19H1K1 | 29H2K2 | 30H3K1 | 41H1K3 |
|---|---|---|---|---|---|---|
| HEK293E cell baseline detection* (WT vs. PINK1 KO) | 2.1 ± 0.02 | 1.3 ± 0.03 | 1.8 ± 0.01 | 1.1 ± 0.04 | 1.4 ± 0.1 | 1.1 ± 0.07 |
| HEK293E cell stressed condition* (WT vs. PINK1 KO) | 143.5 ± 5.9 | 52 ± 0.6 | 91.2 ± 5.7 | 5.6 ± 1.1 | 80.2 ± 6.7 | 4.8 ± 0.2 |
| Mouse brain baseline detection* (WT vs. pink1 KO) | 1.5 ± 0.3 | 1.1 ± 0.1 | 1.6 ± 0.2 | 1.0 ± 0.1 | 1.2 ± 0.1 | 1.0 ± 0.1 |
| Human brain disease condition# (AD vs. control) | 9.3 ± 0.9 | 10.8 ± 0.5 | 2.6 ± 0.2 | 4.9 ± 0.3 | 3.7 ± 0.2 | 2.0 ± 0.1 |
* = ratio of the means is used for cells and mouse tissue, # = ratio of the medians is used for human tissue. AD – Alzheimer disease, KO – knockout, ref. Ab – reference antibody, WT – wild-type.