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. 2004 Dec 14;385(Pt 1):1–10. doi: 10.1042/BJ20041107

Figure 1. Poor inhibition of caspase-9 by ILP2 derivatives.

Figure 1

ILP2 proteins were expressed and purified, and their degree of purity analysed in SDS/PAGE (A). The proteins were titrated against ΔCARD caspase 9 and residual activity quantified as RFU (relative fluorescence units)/min by release of AFC from the caspase substrate Ac-LEHD-AFC (B and C). Replots according to the relationship described in the Experimental section allows calculation of the apparent Ki from the reciprocal of the slopes of the lines in (D) and (E).