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. 2004 Dec 14;385(Pt 1):11–20. doi: 10.1042/BJ20041108

Figure 3. Anti-apoptotic effect of BIR domain constructs.

Figure 3

MCF7 cells were transiently transfected with the reporter plasmid pCMV-βgal and either vector control alone or BIR domain constructs. Following transfection, cells were exposed to doxorubicin (adriamycin; 0.5 μg/ml), stained with X-gal and apoptosis assessed by counting live and dead transfected cells. The percentage apoptosis represents the mean±S.D. for at least four sample points and is representative of three independent experiments. Asterisks designate that the difference between these two values is statistically significant (P<0.05). The relative transfection levels were evaluated by immunoblotting with anti-FLAG antibody.