Figure 3. Complementation of the growth defect of spe1 deleted yeast cells by human ODC.
Wild-type yeast cells (strain Y10000) were transformed with the control plasmid p413ADH (+), isogenic spe1 deleted cells (strain Y15034) were transformed with either the control plasmid p413ADH (O) or plasmid p413ADH-ODC carrying human ODC behind the yeast ADH1 promoter (Δ). Yeast cell growth in polyamine-free, selective glucose medium was followed by determination of A600. The data shown are representative of several experiments.