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. 2004 Dec 14;385(Pt 1):21–28. doi: 10.1042/BJ20040547

Table 1. Nucleotide sequence of primers and vectors used for cloning of ODC, antizymes and AZI.

Protein Primer Cloned into vector
ODC Sense: 5′-GGAGAATTCATGAACAACTTTGGTAATGAAGAGTTTGACTGC-3′ pEG202 p413GALL p413ADH pcDNA3.1(+)
Antisense: 5′-TAGCTCGAGCTACACATTAATACTAGCCGAAGCACAGGCTGC-3′
AZ1 Sense: 5′-GAGGAATTCATGGTGAAATCCTCCCTGCAGCG-3′ pJG4-5, pEG202, p426ADH, p426GAL1, p426GPD
Antisense: 5′-GCACTCGAGCTACTCCTCCTCCTCTCCCGAAGACTCTCTC-3′
Overlap extension: (A) 5′-GGTGCTCC-GATGCCCCTCACC-3′
(B) 5′-GGTGAGGGGCATC-GGAGCACC-3′
AZ2 Sense: 5′-GAGGAATTCATGATAAACACCCAGGACAGTAGTATTTTGCC-3′ pJG4-5 pEG202 p426GAL1 p426GPD
Antisense: 5′-GCACTCGAGTTAGTCCTCATCGGACAAGTTCTGGTC-3′
Overlap extension: (A) 5′-GTGGTGCTCC-GATGCCCCTCAC-3′
(B) 5′-GTGAGGGGCATC-GGAGCACCAC-3′
HA tag, sense: 5′-GGAGAATTCATGTACCCTTATGATGTGCCAGATTATGCCTCT pcDNA3.1(+)
ATAAACACCCAGGACAGTAGTATTTTGCC-3′
AZ3 Sense: 5′-GAGGAATTCATGCTGCCTCGTTGTTATAAAAGCATC-3′ pJG4-5 pEG202 p426GAL1 p426GPD
Antisense: 5′-GCACTCGAGTCAAGGAGGCTCACTGGGCAGG-3′
Overlap extension: (A) 5′-TCCAGTGCTC-TGAGTCCCTAG-3′
(B) 5′-CTAGGGACTCA-GAGCACTGGA-3′
AZ4 Sense: sense primer antizyme 1 pJG4-5 pEG202 p426GPD
Antisense: 5′-GCACTCGAGCTAGCCCTGCAGCGAGTAGG-3′
Overlap extension: (A) 5′-CGGTGGTGCTC-TGATGTCCCT-3′
(B) 5′-AGGGACATCA-GAGCACCACCG-3′
AZI Sense (EcoRI): 5′-GGAGAATTCATGAAAGGATTTATTGATGATGCAAACTACTCCG-3′ pJG4-5 p415GALL p415GPD
Sense (SpeI): 5′-GGAACTAGTATGAAAGGATTTATTGATGATGCAAACTACTCCG-3′
Antisense: 5′-TAGCTCGAGTTAAGCTTCAGCGGAAAAGCTGTCTTCTTGCG-3′
Myc tag, sense: 5′-GATGGATCCATGGAACAGAAGTTGATTTCCGAAGAAGACCTC pcDNA3.1(+)
ATGAAAGGATTTATTGATGATGCAAACTACTCCG-3′