Table 2. The primers’ names and sequences used in the experiment.

Name	Sequence (5′–3′)
pgRNA F primer	5′-TCCCTCGCCTCGCAGACG-3′
pgRNA R primer	5′-GTTTCCCACCTTATGAGTC-3′
β-actin F primer	5′-TTCTACAATGAGCTGCGTGTG-3′
β-actin R primer	5′-GGGGTGTTGAAGGTCTCAAA-3′

pgRNA product length (101 bp): TCCCTCGCCTCGCAGACGAAGGTCTCAATCGCCGCGTCGCAGAAGATCTCAATCTCGGGAATCTCAA TGTTAGTATCCCTTGGACTCATAAGGTGGGAAAC.