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. 2004 Dec 14;385(Pt 1):145–153. doi: 10.1042/BJ20040773

Figure 4. Activation of apo-ZiPD with zinc.

Figure 4

(A) Apo-ZiPD was incubated with zinc concentrations ranging from 0 to 10 mM as described in the Experimental section. Phosphodiesterase activity was measured with 14 mM bpNPP (solid line) in triplicate; data points designate the mean value, error bars designate the S.D. ZiPD concentration during the incubation was 0.093 mg/ml. In a second series (broken line), phosphodiesterase activity was measured with a 14 mM bpNPP solution containing 10 mM EDTA to remove free metal ions (30 min preincubation at room temperature). (B) Zinc activation of ZiPD as isolated, using a lower ZiPD concentration of 0.008 mg/ml. ZiPD is isolated with small, substoichiometric amounts of zinc (0.3 zinc/monomer [11]).