Table 2. Zinc-binding properties of ZiPD, FlRd-lactamase domain and its E81H mutant as determined by ITC.
ZiPD | FlRd-lactamase domain | FlRd-lactamase domain, E81H | |
---|---|---|---|
Kd1 (μM) | 2.2±0.2 | 0.7±0.1 | 30±4 |
ΔH1 (cal/mol) | −6511±90 | −7607±71 | −15990±1126 |
ΔS1 [cal/(mol·deg)] | 3.9 | 2.5 | −33.5 |
ΔG1 (cal/mol) | −7661 | −8345 | −6107 |
Kd2 (μM) | 23.0±0.6 | 26.0±0.1 | 23±2 |
ΔH2 (cal/mol) | −12090±158 | −12000±142 | −4008±1297 |
ΔS2 [cal/(mol·deg)] | −19.8 | −19.7 | 7.6 |
ΔG2 (cal/mol) | −6249 | −6189 | −6250 |