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. 2004 Dec 14;385(Pt 1):207–216. doi: 10.1042/BJ20040162

Figure 6. Mutating the SRE in the proximal SREBP-1c promoter reduces the stimulatory effect of insulin, but not TO901317.

Figure 6

(A) Rat hepatocytes were transfected with pSREBP(−1516/+40)-luc or pSREBP(−1516/+0)-mutSRE-luc and pRL-CMV. Photinus and Renilla luciferase activities were measured after incubation for 24 h in medium with no addition or supplemented with insulin (100 nM). The results are expressed as the means±S.E.M. for eight independent experiments. (B) Rat hepatocytes were transfected with pSREBP(−1516/+40)-luc, pSREBP (−1516/+0)-mutSRE-luc, pSREBP(1516/+40)-mutLXR1/mutLXR2-luc or pSREBP(1516/+40)-mutLXR1/mutLXR2/mutSRE-luc, together with pRL-CMV. Photinus and Renilla luciferase activities were measured after 24 h incubation in medium with no additions or supplemented with insulin (100 nM) or TO901317 (10 μM) or insulin plus TO901317.