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Assays were conducted using the standard assay mixture (pH 7.9, 25 °C), except that the diphosphonicotinamide nucleotide concentration was varied.
| Nicotinamide nucleotide | Vmax (units·mg−1) | Km (mM) | Vmax/Km (units·mg−1·mM−1) |
|---|---|---|---|
| NADH | 41.2±0.9 | 0.042±0.001 | 981 |
| NAD+ | 23.2±0.6 | 2.39±0.05 | 10 |
