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. 2004 Dec 14;385(Pt 1):255–264. doi: 10.1042/BJ20041125

Figure 2. Biophysical characterization of EnvZper.

Figure 2

(A) Far-UV CD analysis of 100 μM refolded EnvZper in 5 mM sodium acetate, pH 5, and 50 mM NaCl; 5 mM potassium phosphate, pH 6, and 50 mM NaCl; 5 mM potassium phosphate, pH 7, and 50 mM NaCl; and 5 mM potassium phosphate, pH 8, and 50 mM NaCl. (B) Near-UV CD analysis of 300 μM native (5 mM sodium acetate, pH 5, and 50 mM NaCl) and denatured (3 M guanidinium chloride and 50 mM NaCl) EnvZper. (C) Intrinsic tryptophan fluorescence analysis of 50 μM EnvZper at pH 5–8. (D) Thermal stability of EnvZper as measured by a change in molar ellipticity at 222 nm. Tm, melting point.