Figure 5. Dimerization of EnvZ_per in solution.

(A and B) Chromatogram of size-exclusion analysis of 100 μM full-length EnvZ_per and CT domain on a Superdex 75 column respectively. The elution volume of protein standards is indicated on top of the chromatogram. (C and D) SDS/12% PAGE analysis of pull-down assays of His₆-tagged EnvZ_per and UT-EnvZ_per at pH 5. The assays in (C) and (D) were performed at pH 5 at various NaCl concentrations. The assays were performed on the following samples: lane 2, His₆–EnvZ_per; lane 3, UT-EnvZ_per; lanes 4–9, His₆–EnvZ_per and UT-EnvZ_per at the indicated NaCl concentrations. (E) SDS/PAGE analysis of pull-down assay of EnvZ_per in the presence and absence of CHAPS at various pHs. (F) Representative sedimentation equilibrium data obtained for 50 μM EnvZ_per at pH 8 in the presence and absence of 10 mM CHAPS. Only the data at 40000 rev./min is represented.