Table 1. Spectral characteristics of the three fluorescent probes presented in Figure 2.

Probe type	Bleaching coefficient, β*	Area of normalized spectra, w (a.u.)†	Maximum extinction coefficient, εM(M−1·cm−1)	Quantum yield, Q	Overlap integral with YFP, J (nm4)‡
CFP	0.90	69	29250±4600§	0.40§	2.21×1010
GFP	0.76	43	53500±2100∥	0.64∥	3.47×1010
YFP	0.19	39	82600±1900¶	0.61¶	−

* Obtained in this work after 60 scans at 514 nm in the confocal microscope.

† Corresponding to the normalized spectra in Figure 2.

‡ See Appendix B for details of the calculation.

§ From [37,38].

∥ From [32,37].

¶ From [37,38,50].