Figure 1. Western blot analysis using antibodies 5/6/3-B-3, and anti-versican in fresh tissue and ligament explant cultures.

Proteoglycans were isolated using anion-exchange chromatography, and the deglycosylated core protein peptides were resolved on 4–15% gradient polyacrylamide/SDS gels, electro-eluted on to PVDF membranes and immunodetected with either 5/6/3-B-3 antibody (lanes a–c: a, fresh ligament tissue; b, cultured tissue; and c, pooled culture medium) or anti-versican (lanes d–f: d, fresh ligament tissue; e, cultured tissue; and f, pooled culture medium).