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. 2005 Jan 7;385(Pt 2):381–388. doi: 10.1042/BJ20040844

Figure 2. Western blot analysis of aggrecan in fresh tissue and ligament explant cultures.

Figure 2

PVDF membranes containing peptides as described in the legend to Figure 1 were immunodetected with either 6-B-4 antibody (lanes a–c: a, fresh ligament tissue; b, cultured tissue; and c, pooled culture medium) or BC-3 (lanes d–f: d, fresh ligament tissue; e, cultured tissue; and f, pooled culture medium). Other membranes containing core protein peptides, isolated from fresh tissue and corresponding to the large proteoglycan peak eluted from the Sephadex CL-4B column, were immunodetected with 1/20/5-D-4 (lane g), 5/6/3-B-3 (lane h) or JD5 (lane i).