Table 2. N-terminal amino acid sequence residues of bovine decorin and biglycan derived from fresh ligament tissue and culture medium.

Deglycosylated core protein peptides from ligament explant cultures were resolved on 4–15% gradient polyacrylamide/SDS gels and electro-eluted on to PVDF sequencing membranes. The bands were cut out and subjected to N-terminal amino acid sequencing. The approx. M_r values of peptides with the corresponding yield of the first amino acid in the peptide sequence respectively are shown. ‘X’ in the amino acid sequences represents an unidentified amino acid residue.

Fresh tissue matrix peptides			Culture medium peptides
Approx. Mr (×10−3)	Residues	Yield (pmol)	Approx. Mr (×10−3)	Residues	Yield (pmol)	Origin of peptide sequence
43	DEAXGIGP	500	43	DEASGIGP	115	D1EASGIGP*
32	DEAXGIGP	27	32	DEAXGIGP	5
30	DEAXGIGP	12	30	DEAXGIGP	11
21	DEAXGIGP	42	21	DEAXGIGP	16
18	DEAXGIGP	51	18	DEAXGIGP	119
13	DEAXGIGP	20	13	DEAXGIGP	13
			40	DEEAXGAE	22	D1EEASGAE†

* Peptide sequence is based on the primary sequence of bovine decorin [36].

† Peptide sequence is based on the primary sequence of bovine biglycan [36].