Skip to main content
. 2005 Jan 7;385(Pt 2):427–432. doi: 10.1042/BJ20041218

Figure 1. Affinity purification of EC-SOD.

Figure 1

(A) Plasma from a wild-type individual (25 ml) and a homozygous R213G individual (5 ml) was applied to a heparin–Sepharose column and bound proteins were subsequently eluted with NaCl (indicated by broken line). Collected fractions were analysed for the presence of EC-SOD by using a heparin-based ELISA assay. The amount of EC-SOD is given as the absolute absorbance. (B) Collected fractions were analysed by SDS/PAGE using 600 and 40 μl of fractions representing wild-type and R213G plasma respectively. EC-SOD was subsequently detected by using Western blotting. The intact, intermediate and cleaved forms of EC-SOD are indicated. C, wild-type EC-SOD purified from human aorta used as control.