Figure 3.

PlaB reduces the level of pre-, but not mature, miR-122 and miR-17. (A) Total RNA was extracted from Huh7 cells ± PlaB. Mature miR-122 was analysed by RT-qPCR, normalized to U6 snRNA and shown relative to a DMSO-treated control. Data represent mean of three independent experiments, with error bars showing SD. n.s. indicates not significant. (B) Total RNA extracted from Huh7 cells ± PlaB was size fractionated and the resulting small RNA was analysed by northern blot. ³²P end-labelled oligonucleotide probes specific to the indicated mature miRNA sequences were used to detect pre- and mature miRNAs over successive probing of the same membrane. U6 snRNA was used as a loading control. Images shown are representative of at least three independent experiments.