Figure 1. Representative calorimetric isotherms of binding of CcCBM17 (A) and BspCBM28 (B) to RC at 25 °C and 15 °C respectively in 50 mM potassium phosphate, pH 7.0.

The upper panels show the raw data, and the lower panels the integrated data. The CcCBM17 and BspCBM28 titrations into RC are shown as solid circles. The lines show the fits to a two independent binding site model. The corresponding heat of dilution data (CBM titrated into buffer) are shown for reference as open circles. 1 kcal=4.184 kJ.