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. 2005 Jan 7;385(Pt 2):493–502. doi: 10.1042/BJ20041160

Figure 1. Detection of LPPs by RT-PCR and Western blotting in HBEpCs.

Figure 1

(A) Total RNA was extracted from HBEpCs and transcription of the genes encoding LPPs (LPP-1, -2 and -3, and SPP-1) was assessed by RT-PCR (− indicates the absence of reverse transcriptase and + indicates the presence of reverse transcriptase during the RT reaction) with primers to the indicated LPPs. Lane M contains molecular size markers. (B) Cell lysates (20 μg of protein) were subjected to SDS/PAGE and analysed by Western blotting with anti-LPP-1, -LPP-2 and -LPP-3 antibodies. Each Western blot is representative of three independent experiments.