Table 2. Effects of overexpression of Myc-tagged mLPP-1 on LPA-, PMA- and TNF-α-induced IL-8 secretion in HBEpCs.
HBEpCs (∼70% confluence in 35 mm dishes) were infected with vector control or Myc-tagged mLPP-1 wt adenoviral constructs (MOI 10; 2×105 cells per dish) for 24 h. Cells were challenged with 1 ml of medium alone or containing LPA (1 μM), PMA (25 nM) or TNF-α (20 ng/ml) for 3 h. The supernatants were collected and centrifuged, and aliquots stored at −80 °C for analysis of IL-8 by ELISA as described in the Experimental section. Values are means±S.D. of three independent experiments in triplicate, and are expressed either as pg/μg of protein in cell lysates or % of control.
| IL-8 | ||
|---|---|---|
| Treatment | (pg/μg of protein) | (% of control) |
| Vehicle | 1.2±0.01 | 100 |
| LPA (1 μM) | 16.3±2.6 | 1358 |
| PMA (25 nM) | 29.4±3.9 | 2450 |
| TNF-α (20 ng/ml) | 11.6±2.4 | 967 |
| mLPP-1 wt+vehicle | 1.1±0.2 | 92 |
| mLPP-1 wt+LPA (1 μM) | 4.2±0.6 | 350 |
| mLPP-1 wt+PMA (25 nM) | 24.2±2.3 | 2017 |
| mLPP-1 wt+TNF-α (20 ng/ml) | 6.8±0.4 | 567 |