Table 1. Subcellular distribution of AP-4.
Immunogold particles were quantified on single-labelled cryosections from MDCK and MelJuSo cells. Gold particles were scored under the microscope and assigned to Golgi complex, transport vesicles and endocytic compartments. The total numbers of gold particles were: anti-ε in MDCK cells, 240; anti-μ4 in MDCK cells, 427; anti-μ4 in MelJuSo cells, 311.
| Immunogold labelling (% of total particles) | |||
|---|---|---|---|
| Compartment | Anti-ε in MDCK | Anti-μ4 in MDCK | Anti-μ4 in MelJuSo |
| Golgi complex | 33 | 39 | 38 |
| Vesicles | 56 | 45 | 47 |
| Close to early endosomes | 16 | 20 | 10 |
| Close to late endosomes | 11 | 8 | 17 |
| Others | 29 | 17 | 20 |
| Endosomes | 11 | 16 | 15 |
| Early | 9 | 13 | 6 |
| Late | 2 | 3 | 9 |