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Immunogold particles were quantified on clathrin- and μ4-double-labelled cryosections from MDCK cells. Only gold particles found on transport vesicles were scored in random micrographs at ×39000. A vesicle was considered positive when a minimum of one gold particle was found.
| Immunogold-labelled proteins | Number of positive vesicles |
|---|---|
| Clathrin | 161 |
| μ4 | 158 |
| Clathrin plus μ4 | 37 |
