Table 1. Processivity of Cel7A on cellulose substrates with different average DPs.
n.d., not determined.
| AA labelling method | Somogyi–Nelson method | ||||
|---|---|---|---|---|---|
| Substrate | Reducing end groups (μmol/g) | DP (glucose units) | Reducing end groups (μmol/g) | DP (glucose units) | Processivity of Cel7A (glucose units)* |
| BMCC | 39±0.3 | 150±2 | 48±1† | 130±3 | 84±20 |
| Amorphous BMCC | 37±2 | 163±9 | 46±1‡ | 131±3 | n.d. |
| CM-cellulose | 21±5 | 300±72 | 20±2 | 300±30 | n.d. |
| EG-BC | 10±1 | 600±60 | n.d. | n.d. | 68±4 |
| BC | 3.5±0.5 | 1700±25 | n.d. | n.d. | 176±20 |
* Measured on AA-labelled substrates according to eqn (5).
† Measured at a cellulose concentration of 5 mg/ml.
‡ Measured at a cellulose concentration of 8 mg/ml.