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. 2005 Jan 7;385(Pt 2):557–564. doi: 10.1042/BJ20040684

Figure 3. Identification of the Sp1-binding sites responsible for minimal promoter activities of DNMT3A and DNMT3B in HEK-293T cells.

Figure 3

Left panels: schematic structure of the minimal promoter of DNMT3A 3rd promoter pGL3A-P3 (−334/+376) (A), DNMT3B 1st promoter pGL3B-P1 (−102/+309) (B), DNMT3B 2nd promoter pGL3B-P2 (−469/+260) (C) and their deletion constructs are illustrated. The Sp1-binding sites are presented as open boxes and positions are shown relative to the TSP (+1) of each exon. Right panels: the plasmids containing minimal promoter of DNMT3A 3rd promoter, DNMT3B 1st and 2nd promoters and their deletion mutants were transfected into HEK-293T cells. Luciferase activity was expressed relative to the activity of empty reporter vector pGL3-basic. Results are expressed as the means±S.D. for three independent experiments.