Skip to main content
. 2005 Jan 7;385(Pt 2):581–588. doi: 10.1042/BJ20041388

Figure 3. SSO1949 degrades CMC and cello-oligomers.

Figure 3

(A) A 1% CMC solution was incubated with 1.1 μM SSO1949 at pH 1.8 and 80 °C. Samples were withdrawn at several time points, and the viscosity (○) and the amount of reducing ends (●) were measured. (B) Cellobiose (C2), cellotriose (C3), cellotetraose (C4), cellopentose (C5) and a hydrolysate of cellulose (H), each 8 mg/ml, were incubated with 1.3 μM SSO1949 in 20 mM potassium phosphate (pH 1.8) for 60 min at 80 °C. Reactions with (+) and without (−) enzyme were spotted on silica 60 plates and developed with a mixture of ethyl acetate/acetic acid/water (2:1:1, by vol.). Sugars were visualized with thymol sulphuric acid. Only oligomers larger than three units are degraded. Cellobiose and cellotriose are the main products of enzymic hydrolysis by SSO1949.