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. 2005 Jan 24;385(Pt 3):625–637. doi: 10.1042/BJ20041361

Figure 1. Resonance energy transfer.

Figure 1

RET occurs between donor and acceptor molecules if they are in close proximity (less than 100 Å), resulting in energy emission from the acceptor at a characteristic wavelength. FRET involves excitation of a donor fluorophore with light (A), whereas BRET occurs when the donor Rluc catalyses the oxidation of coelenterazine to coelenteramide (B). Both of these techniques are particularly suitable for investigating GPCR–protein complexes in living cells, including oligomerization (C). Illustration created by Uli Schmidt (http://www.scigraphico.com.au/).