Figure 1. Generation of mice lacking PDK1 in hepatocytes.

(A) Breeding strategy used for the generation of mice lacking PDK1 in hepatocytes, where AlfpCre denotes transgenic expression of the Cre recombinase under the albumin promoter [21]. Throughout the present study, PDK1^{flΔneo/flΔneo} AlfpCre^+/− mice are termed L-PDK1^−/− and PDK1^{flΔneo/flΔneo} AlfpCre^−/− mice are termed L-PDK1^+/+. (B) PDK1 was immunoprecipitated from 500 μg of liver extracts, and kinase activity was measured as described in the Materials and methods section. Each point represents the mean activity±S.D. of three different livers, with each assayed in triplicate. For the PDK1 immunoblot analysis, 10 μg of brain (br), 20 μg of adipose tissue (ad), 25 μg of skeletal muscle (mu) and 30 μg of liver (li) extract were used.