Figure 3. Activation of PKB in liver by insulin.

Mice were fasted overnight and injected with either saline for 10 min (for the zero time point control) or insulin (1 m-unit/g of body mass) for the indicated times. The liver was rapidly extracted and frozen in liquid nitrogen. (A) PKB was immunoprecipitated from liver extracts, and the activity was determined using a quantitative peptide phosphorylation assay. Each point represents the mean activity±S.D. of three different livers with each assayed in triplicate. (B) Lysates were also analysed by immunoblot analysis with the indicated antibodies. Every lane corresponds to extracts isolated from individual mice.