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. 2005 Jan 24;385(Pt 3):649–657. doi: 10.1042/BJ20040829

Figure 5. Outward current in excised patches.

Figure 5

The bath (pH 7.0) and pipette (pHo 8.0) solutions were as described in the Materials and methods section. Excised patches were detached from CHO cells expressing EGFP-C–91 following formation of a GΩ seal. The outward currents (B) were recorded immediately after excision, in response to the voltage step protocol (A). The reversal potential of the outward current in a second excised patch was determined following an activation pulse to +100 mV (C). The first-order exponential fit of the tail currents is overlaid in grey (C). The amplitude of the tail currents (−20 mV to −80 mV) was plotted against the voltage of the repolarization step (D).