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. 2005 Jan 24;385(Pt 3):745–754. doi: 10.1042/BJ20041015

Figure 5. Absorption (A) and EPR (B) spectra of T. hirsuta laccase during the redox titration by different mediators.

Figure 5

The experiments were performed in 0.1 M phosphate buffer, pH 6.5. Spectrum E characterizes the solution of laccase before addition of mediators. Spectrum 1 was recorded after mixing the enzyme with K3Mo(CN)8 and K4Mo(CN)8 (the redox potential after equilibrium, 770 mV versus NHE). Spectrum 2 was recorded after mixing the enzyme with K3Fe(CN)6 and K4Fe(CN)6 (the redox potential after equilibrium, 450 mV versus NHE).