Figure 2. Separation of loosely bound [¹⁴C]NADPH from catalase by chromatography on a column of G-25.

The distributions are those obtained in the experiment of Figure 1. ×, catalase after exposure to unlabelled NADPH; ○, catalase after exposure to [¹⁴C]NADPH; ●, [¹⁴C]NADPH. The ratio of nmol of [¹⁴C]NADPH bound per nmol of catalase was 0.07 in the solution pooled from tubes containing most of the catalase (tubes 11–14).