Figure 2. Effects of fatty acids on the expression of genes involved in cholesterol synthesis and in peroxisomal fatty acid β-oxidation, and on FPP synthase protein expression, in H4IIEC3 rat hepatoma cells.

Northern blot analysis of the expression of FPP synthase, HMG-CoA reductase (A) and AOX (B) after fatty acid treatment for 18 h. mRNA levels were normalized to those of 36B4 mRNA. mRNA levels relative to BSA control treatments (determined by densitometry) are indicated. One Northern blot analysis representative of five independent experiments is shown. (C) Western blot analysis of FPP synthase protein levels after treatment with 50 μM or 100 μM fatty acid for 18 h, normalized to HSC70 protein levels. Protein levels relative to BSA control treatment (determined by densitometry) are indicated (data from one experiment representative of two independent experiments).