Table 1. Identification of the FSAP cofactor from cell-conditioned medium.
Conditioned medium from CHO-745 cells was treated as indicated and subsequently tested for FSAP cofactor activity as compared with buffer control (representing 100%) containing each of the additives indicated. The results are representative of one experiment out of four.
Biomolecule classification | Treatment of cell-conditioned medium | FSAP activity (% of control) |
---|---|---|
Protein | Protease inhibitors | 350 |
Heat denaturation | 350 | |
Trypsin, soya-bean trypsin inhibitor | 450 | |
Carbohydrate | Glycosidase | 200 |
Neuraminidase | 250 | |
Lipid | Butanol extract | 200 |
Methanol/chloroform extract | 200 | |
Nucleic acid | Dnase | 450 |
RNase A | 100 |